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PCR-based assays

 

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Study report: Thermo Scientific SureTect E. coli O157:H7 PCR Assay Method Extension For Use With the Applied Biosystems QuantStudio 5 RealTime PCR Instrument AOAC-RI PTM Validation: Method Comparison Study

16 September 2020 | By

The data presented in this report show that the SureTect E. coli O157:H7 PCR Assay is suitable for the detection of E. coli O157:H7 from a variety of food samples when using the QuantStudio 5 Real-Time PCR Instrument and associated RapidFinder Analysis Software.

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Study report: Thermo Scientific SureTect Salmonella Species PCR Assay Method Extension For Use With the Applied Biosystems QuantStudio 5 Real-Time PCR Instrument AOAC-RI PTM Validation: Method ComparisonStudy

15 September 2020 | By ,

The data presented in this report show that the SureTect Salmonella species PCR Assay is suitable for the detection of Salmonella species from a variety of food and environmental surface samples when using the QuantStudio 5 Real-Time PCR Instrument and associated RapidFinder Analysis Software.

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Study report: Thermo Scientific SureTect Listeria species PCR Assay and Thermo Scientific SureTect Listeria monocytogenes PCR Assay method extension for use with the Applied Biosystems QuantStudio 5 RealTime PCR Instrument AOAC-RI PTM Validation: Method Comparison Study

15 September 2020 | By

This report details the method comparison study between the candidate methods and the International Organisation for Standardisation (ISO) 112901:2017 for a representative range of matrices.

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Loop-mediated isothermal nucleic acid amplification (LAMP) for food microbiology testing

2 April 2017 | By ,

Contamination of food products with pathogenic microorganisms is a major concern for food safety. The issue is exacerbated by modern production systems where high volumes, high speed and global distribution are common features. In order to protect consumers, regulations and procedures are in place to control microbiological hazards in the…

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Appropriate controls for PCR detection of foodborne pathogens

30 April 2012 | By Martin D’Agostino, Microbiologist, The Food and Environment Research Agency

Over the years, there has been a great increase in the number of PCR based assays for foodborne pathogen detection. For example, a very basic search for ‘salmonella food PCR assay’ using the PubMed.gov database will produce over 600 results. Clearly, this has led to a huge choice of PCR-based…