Study report: Thermo Scientific SureTect Listeria species PCR Assay and Thermo Scientific SureTect Listeria monocytogenes PCR Assay method extension for use with the Applied Biosystems QuantStudio 5 RealTime PCR Instrument AOAC-RI PTM Validation: Method Comparison Study

This report details the method comparison study between the candidate methods and the International Organisation for Standardisation (ISO) 112901:2017 for a representative range of matrices.

Thermo Scientific^™ SureTect^™ Listeria monocytogenes PCR Assay and Thermo Scientific^™ SureTect^™ Listeria species PCR Assay (candidate methods) have been validated in accordance with the AOAC Research Institute Performance Tested MethodsSM (PTM) Program for the detection of Listeria from a variety of food and environmental surfaces. The candidate methods have been validated for use with the Applied Biosystems^™ QuantStudio^™ 5 Real-Time PCR Instrument to perform PCR and Thermo Scientific RapidFinder^™ Analysis Software v1.0 or greater for data analysis.