Lipid and fatty acid analysis using state-of-the-art gas chromatography
The characterisation of lipids is one of the major applications of gas chromatography in food analysis. In general, GC can be applied to the analysis of intact lipids, such as triglycerides, or to the analysis of the fatty acid composition based on fatty acid methyl ester (FAME) profiling.
A broad range of columns and stationary phases are available for FAME analysis. In this presentation, some column technologies will be presented and the GC column selection will be discussed.
Based on the complexity of the samples, different column technologies can be applied and a trade-off can be made between sample throughput and resolution.
It is also demonstrated that using state-of-the-art GC equipment, lab productivity can substantially be increased by optimisation of heating, cooling and column exchange cycles.
Besides the analysis of fatty acid composition, also the analysis of intact lipids, such as triglycerides and wax esters can be performed by GC. High temperature GC conditions and dedicated GC columns are hereby required.
Examples will be given for carbon number separation and for detailed analysis of triglycerides using more polar, thermally stable columns.
Finally, some remarks will be given on sample preparation.
What you will learn:
- An overview of GC solutions for lipid analysis will be given
- Attention will be paid to column selection for the analysis of fatty acid methyl esters with focus on resolution versus sample throughput
- Fast GC analysis using state-of-the-art instrumentation will be presented
- The potential and limitation of high temperature GC for the analysis of intact lipids will be discussed.
Frank David Ph.D, R&D Director, Research Institute for Chromatography (R.I.C)
Dr. Frank David received his PH.D degree in 1986 at the Laboratory of Organic Chemistry of the Universiteit of Gent under the direction Prof. Dr. M. Verzele. Since October 1986, Frank David is R&D manager at the Research Institute for Chromatography (RIC) in Kortrijk under the direction of Prof. Dr. Pat Sandra.
Frank David is author of more than 100 scientific papers in different areas of separation science. His expertise includes capillary gas chromatography, supercritical fluid chromatography (SFC), GC-mass spectroscopy (GC-MS), liquid chromatography (HPLC), liquid chromatography-mass spectroscopy (LC-MS), multidimensional chromatography (GC-GC, GCxGC, LC-GC) and miniaturisation and automation of sample preparation.
Frank David is also consultant for instrument manufactures and for industrial laboratories in environmental, petrochemical, food, pharmaceutical and chemical industries. In this function, he has a long experience in theoretical and practical training courses.