Whitepaper: Quantitative PCR analysis of meat speciation data using the 2-∆∆ Cq method
22 June 2015 • Author(s): Primerdesign
In recent years the food production industry has been placed under intense scrutiny. This has led to an increased pressure for advancements in the field of speciation testing. Quantitative PCR is a highly sensitive and accurate method of assaying samples for nucleic acid content. In combination with well-established DNA extraction procedures, PCR is now being used by many labs to quantify relative levels of animal DNA in total meat. Using the 2^-∆∆Cq method, calculations can be accurately applied to multiplex formats of speciation tests (including two sets of primer and probe, one for the species of interest, the other as a universal detection system), using total DNA as a positive control calibrator sample.
Find out more about Primerdesign’s complete speciation solution, using the most up-to-date methods, as part of the genesig® range of products for accurate and accessible speciation testing.
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