Automated SPE-LC-MS/MS method for Acrylamide in Coffee
Acrylamide has been labelled as a probable human carcinogen. Due to the use of roasted coffee beans in making coffee and the high consumption of coffee world-wide, brewed coffee could be a source of daily exposure to acrylamide. Acrylamide determination has been shown to be challenging due to presence of coextractives in the final extract. Manual solid phase extraction followed by LC-MS/MS analysis has been reported as a successful method for the determination of acrylamide from brewed coffee samples. However, performing solid phase extraction manually can be tedious and time-consuming and there is increasing demand for automation of these methods.
In this study, we show that a manual SPE method used for the determination of acrylamide in brewed coffee can be converted to an autosampler-compatible cartridge format and automated using a robotic autosampler controlled by user-friendly software.
The rest of this content is restricted - login or subscribe free to access
Thank you for visiting our website. To access this content in full you'll need to login. It's completely free to subscribe, and in less than a minute you can continue reading. If you've already subscribed, great - just login.
Why subscribe? Join our growing community of thousands of industry professionals and gain access to:
- bi-monthly issues in print and/or digital format
- case studies, whitepapers, webinars and industry-leading content
- breaking news and features
- our extensive online archive of thousands of articles and years of past issues
- ...And it's all free!
Related content from this organisation
- Application Note: Automated Extraction-SPE-LC-MS of Antibiotics in Egg
- Aroma in High Fat Foods by Thin Film(TF)-SPME-TD-GC/MS
- App Note: VOCs in food packaging by Thermal Extraction-GC/MS
- Sample preparation and analysis solutions for water and edible oil
- Determining phenols in whisky by SBSE-GC/MS & LVI-GC/MS