Automated SPE-LC-MS/MS method for Acrylamide in Coffee
Acrylamide has been labelled as a probable human carcinogen. Due to the use of roasted coffee beans in making coffee and the high consumption of coffee world-wide, brewed coffee could be a source of daily exposure to acrylamide. Acrylamide determination has been shown to be challenging due to presence of coextractives in the final extract. Manual solid phase extraction followed by LC-MS/MS analysis has been reported as a successful method for the determination of acrylamide from brewed coffee samples. However, performing solid phase extraction manually can be tedious and time-consuming and there is increasing demand for automation of these methods.
In this study, we show that a manual SPE method used for the determination of acrylamide in brewed coffee can be converted to an autosampler-compatible cartridge format and automated using a robotic autosampler controlled by user-friendly software.
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