Whitepaper/App note

Automated SPE-LC-MS/MS method for Acrylamide in Coffee

Posted: 21 August 2018 | | No comments yet

Acrylamide is thought to be produced during the roasting process associated with coffee production…

Acrylamide has been labelled as a probable human carcinogen. Due to the use of roasted coffee beans in making coffee and the high consumption of coffee world-wide, brewed coffee could be a source of daily exposure to acrylamide. Acrylamide determination has been shown to be challenging due to presence of coextractives in the final extract. Manual solid phase extraction followed by LC-MS/MS analysis has been reported as a successful method for the determination of acrylamide from brewed coffee samples. However, performing solid phase extraction manually can be tedious and time-consuming and there is increasing demand for automation of these methods.

In this study, we show that a manual SPE method used for the determination of acrylamide in brewed coffee can be converted to an autosampler-compatible cartridge format and automated using a robotic autosampler controlled by user-friendly software.

The rest of this content is restricted - login or subscribe free to access

New Food issue 5 2018Thank you for visiting our website. To access this content in full you'll need to login. It's completely free to subscribe, and in less than a minute you can continue reading. If you've already subscribed, great - just login.

Why subscribe? Join our growing community of thousands of industry professionals and gain access to:

  • bi-monthly issues in print and/or digital format
  • case studies, whitepapers, webinars and industry-leading content
  • breaking news and features
  • our extensive online archive of thousands of articles and years of past issues
  • ...And it's all free!

Click here to Subscribe today Login here

 

Send this to a friend