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Application Note: Analysis of sugar alcohols and allulose

Overconsumption of sugar (sucrose) can lead to many health issues such as obesity, diabetes, dental diseases, and ADHD (attention-deficit/hyperactivity disorder). To support the reduction of these problems, many food and beverage manufacturers have reformulated or developed new products with sugar substitutes such as sugar alcohols (polyols) or allulose,

Abstract
Overconsumption of sugar (sucrose) can lead to many health issues such as obesity, diabetes, dental diseases, and ADHD (attention-deficit/hyperactivity disorder). To support the reduction of these problems, many food and beverage manufacturers have reformulated or developed new products with sugar substitutes such as sugar alcohols (polyols) or allulose, an epimer of the monosaccharide sugar fructose, which has a lower glycemic index and calories than sugar when digested. There is no fixed daily intake, but it is important to know the value of sugar alcohols and allulose present in food and pharmaceutical products to prevent overconsumption, due to possible laxative effects from some of the sugar alcohols. An analytical method has been developed to detect and quantify sugar alcohols and allulose in beverages and other low-calorie products. Arc HPLC-RI was combined with an Atlantis Premier BEH Z-HILIC analytical column, which provides an increase in polar analyte retention and offers a different selectivity when compared to other hydrophilic interaction liquid chromatography (HILIC) chemistries. The proposed analytical workflow may be suitable for supporting manufacturers and contract testing laboratories in standardizing analyses for sugar alcohols and allulose in sugar-free or reduced-sugar products.

Benefits

  • Retention and separation of 7 sugar alcohols and allulose using the Atlantis Premier BEH Z-HILIC Column, which is efficient for products using blends of sweeteners
  • Simple isocratic liquid chromatography (LC) method, ease of method setup for routine product quality control
  • Arc HPLC cycle injector valve reduces downtime and method risks due to possible precipitation of samples
  • RI Detection of components without UV chromophores

Excellent reproducibility, accuracy, and precision of Arc HPLC-RI Detector with the Atlantis Premier BEH Z-HILIC Column