Application note: Estimation of beta-sitosterol in milk fat (Ghee) by Agilent 8890 GC and 5977B MS
This application note demonstrates the use of the Agilent 8890 GC and the Agilent 5977B GC/MS single quadrupole mass spectrometer in the detection and quantification of β-sitosterol in milk fat samples to check for vegetable oil adulteration. The method provides the highest confidence for routine analysis of milk fat samples in the food industry, whether it is used in manufacturing, processing, commercial testing, or academia. Sample preparation for this method involved saponification, followed by extraction of unsaponifiable matter by liquid-liquid extraction (LLE) and derivatisation of sitosterol to its trimethylsilyl derivative.
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