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PCR-based assays - Articles and news items

DuPont BAX System real-time PCR assay for Salmonella receives AOAC-RI validation extension

Industry news  •  24 September 2015  •  Victoria White

The AOAC-RI has approved a method extension for the DuPont BAX System real-time PCR assay for Salmonella to include enrichment protocols using Actero Salmonella Enrichment Media from FoodChek Systems…

Viability PCR – the next level in PCR-based pathogen testing

Webinars  •  29 October 2014  •  Dr. Marcia Armstrong

This recorded webinar introduces viability PCR as a fast and powerful tool to analyze food samples for the presence of potentially harmful microbes…

Appropriate controls for PCR detection of foodborne pathogens

Issue 2 2012  •  30 April 2012  •  Martin D’Agostino, Microbiologist, The Food and Environment Research Agency

Over the years, there has been a great increase in the number of PCR based assays for foodborne pathogen detection. For example, a very basic search for ‘salmonella food PCR assay’ using the PubMed.gov database will produce over 600 results. Clearly, this has led to a huge choice of PCR-based detection methods for analysts. This is especially so for analytical laboratories who choose to use non-proprietorial PCR-based methods, as opposed to commercially available complete PCR detection systems.

Since PCR-based assays are based on nucleic acid amplification, they are highly efficient, but they can also be negatively affected by the presence of food matrix-derived substances which can interfere or prevent the reaction from performing correctly. This is the case whether using a commercially available system, or a freely available non-proprietorial published method. Therefore, the use of appropriate and careful sample treatment must be applied or used to remove these inhibitory substances as much as is possible.

It must be noted however, that no sample treatment can be relied on completely, thus a suite of controls should be employed to verify that both the sample treatment and the PCR-based assay has performed correctly.

 

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