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LC-MS - Articles and news items

e-Mag: Top three challenges in external accreditation audits

Whitepapers  •  12 January 2016  •  Romer Labs

In this free magazine for analytical industry professionals, we address the three biggest challenges that labs encounter during the external audit process: traceability, measurement uncertainty and matrix effects…

App Note: Multi-residue analysis of highly polar pesticides in food safety by LC-MS/MS

Whitepapers  •  12 January 2015  •  David R. Baker, Shimadzu, Manchester, UK / Mikaël Levi, Shimadzu, Marne-La-Vallée, France / Eric Capodanno, Phytocontrol, Nimes, France

Food safety laboratories involved in pesticide residue monitoring typically employ a multi-residue LC-MS/MS method for the quantification of an ever increasing list of target pesticides. In this study we present two methods developed for the analysis of a wide range of polar pesticides using the Shimadzu LCMS-8050…

Mass Spectrometry supplement 2012

Issue 6 2012, Supplements  •  13 December 2012  •  José Bernal del Nozal, Pilar Manzano, Juan Carlos Diego, María Jesús Nozal, Carmen Ferrer, Amadeo R. Fernández-Alba

Studying the influence of the potato variety and frying oil on fatty acid content of commercial potato crisps (José Bernal del Nozal, Pilar Manzano, Juan Carlos Diego and María Jesús Nozal – IU CINQUIMA, Analytical Chemistry Group, University of Valladolid)
LC-MS/MS and pesticide residue analysis in food (Carmen Ferrer and Amadeo R. Fernández-Alba – European Union Reference Laboratory for Pesticide Residues in Fruit & Vegetables, Universidad de Almeria)

The detection and screening of mycotoxins by LC-MS/MS

Webinars  •  7 August 2012  •  Dr. Stephen Lock, PhD / Dr. Ondrej Lacina, PhD / Dr. Florian Huebner

This on-demand webinar covers the different approaches to the detection of mycotoxin contamination in food by LC-MS/MS.

Whitepaper: Advanced mycotoxin testing system

Uncategorized  •  1 June 2012  •  ToxiMet

The analytical resource, expertise, cost and time that is invested into the mycotoxin testing of foods is enormous. The ToxiMet system is a simple, affordable, rugged and reliable system that can accurately and rapidly measure mycotoxin levels at every step in the supply chain without the need of a highly trained operator…

LCMS as a reliable tool for monitoring food allergens contamination

Issue 2 2012  •  1 May 2012  •  Linda Monaci, National Research Council of Italy (ISPA-CNR)

Food allergen research has considerably expanded its field of interest in recent years probably due to the increasing incidence of food allergies throughout the population. According to the last legislation issued on this issue, there is a current trend to develop reliable methods tailored to the detection of food allergens for routine-like applications. Lately, MS-based methodologies have attracted the interest of researchers owing to the great potentials offered by this technology, the ability to perform multi-allergen screening in food products. The MS approach is currently being adopted by the allergen detection community, proving to be a valid alternative to ELISA and PCR methods. MS methodologies applied to food allergen detection are herein presented. There has been an increasing interest in the field of food allergy in the last few decades probably due to the wide spread of this pathology throughout the population, especially affecting industrialised countries. A food allergy is defined as an abnormal immunological reaction (Ig-E and non-IgE-mediated) triggered by the ingestion of a food or food additive also known as ‘food allergen’. The mechanisms by which a person develops an allergy to specific foods are largely unknown. However, there is accumulated evidence that the early colonisation of the intestinal tract by an appropriate intestinal microbiota is important for the healthy maturation of the immune system with an appropriate programming of oral tolerance to dietary antigens. Whether an individual becomes sensitised or tolerant to an allergen depends on the timing and dose of the allergen as well as the route of exposure…

Rapid and automated screening of priority β-agonists in urine using high resolution LC-MS technology

Issue 4 2011  •  6 September 2011  •  Thorsten Bernsmann and Peter Fürst, Chemical and Veterinary Analytical Institute, and Michal Godula, Thermo Fisher Scientific

Beta-agonists (β-agonists) are synthetically produced compounds that are widely known for their bronchodilatory and tocolytic effects. At higher doses, these substances also have anabolic effects and can promote live weight gain in food producing animals. Clenbuterol is the most commonly used β-agonist for growth-promoting purposes, despite the fact that there have been documented cases when consumption of liver and meat from animals treated with clenbuterol has resulted in serious human intoxication1. Increased levels of β-agonists and their analogs have been associated with acute toxic effects such as heart palpitation, muscle tremor, tetany and severe migraines.

Due to their adverse effects, the use of β-agonists has been banned by the European Union and other regulatory agencies worldwide. Nevertheless, monitoring programmes have shown that β-agonists are still illegally used by food producers and moreover, newly developed analogs with modified structures are being continuously introduced in routine practice. As a result, there is a clear need for quick and simple screening methods to routinely and accurately monitor levels of β-agonists in samples of animal origin, including urine, plasma and tissues.

Specific migration of plasticisers coming from closure gaskets: The LC-MS direction

Issue 1 2011  •  3 March 2011  •  Michele Suman, Food Chemistry and Safety Research Manager, Barilla Food Research Labs

PVC gaskets seals are used for packaging many food commodities (e.g. sauces, vegetables in oil, baby food) in glass jars with metal twist closures, preventing microbiological contamination and providing an easy opening at the same time. The plastisols consist of poly(vinyl chloride) (PVC) usually containing 25 – 45 per cent of the weight of plasticisers and additives such as epoxidised soybean oil (ESBO), phthalates, sebacates, adipates, citrates, acetylated mono/diglycerides, slip agents and lubricants. Because of low molecular weight and the lack of chemical bonds between most plasticiser molecules and PVC, they can easily be extracted from the polymer matrix.

The major risks for the potential migration of these plasticisers into the food are associated with the packaging and sterilising procedure (when the food is warm and under high pressure), then the migration could continue by occasional contact during transportation and storage.

Using LC-MS to study the fate of food ingredients in the human body

Issue 2 2010  •  12 May 2010  •  Christian H. Grün & Hans-Gerd Janssen, Unilever Research and Development, Advanced Measurement & Data Modelling

The human body is designed for effectively extracting nutrients from the food we eat. The nutrients provide the body with energy, but in addition, they also provide the building blocks for cell growth. More recently, it has also been realised that specific food ingredients can be associated with a direct stimulating effect on our health. Examples are the multiple unsaturated fatty acids and plant sterols which actively reduce blood cholesterol levels. Others are flavonoids; natural antioxidants found at large quantities in tea, red wine, cocoa, fruits and vegetables. For example, a diet rich in flavonoids is associated with reduced risk of cardiovascular disease. In particular, consumption of tea, grape (juice), and red wine has been shown to improve markers of vascular function.

New Food Digital Issue 3 2010

Past issues  •  7 May 2010  •  

In this issue: Simultaneous determination of regulatory mycotoxins using immunoaffinity columns in conjuction with either HPLC or LC-MS/MS, Preparing for the R22 refrigerants ban – Ozone Depleting Substances Regulation: HCFC (R22) phase-out

LC-MS/MS based quantitative methods for multiple mycotoxins in food

Issue 1 2009  •  20 February 2009  •  Michael Sulyok, Rainer Schuhmacher & Rudolf Krska, Centre for Analytical Chemistry, Department for Agrobiotechnology, IFA Tulln, University of Natural Resources and Applied Life Sciences Vienna

Since the introduction of atmospheric pressure ionisation liquid chromatography-mass spectrometry (API-LC-MS) in the early 1990s, there was a continuous effort to further improve the performance of the LC-MS instruments concerning sensitivity and robustness. One result of this development is the trend towards methods that are designed to simultaneously analyse a large number of analytes with little or even without any sample clean-up and/or analyte enrichment. It is evident that this approach exhausts the capabilities of the mass spectrometers to the extreme. In this article, the difficulties that are usually encountered during the development of such a multi-analyte method are discussed using the example of mycotoxins.

A farm to fork approach to lower acrylamide in food

Issue 2 2008, Past issues  •  13 June 2008  •  Frédéric Mestdagh and Bruno De Meulenaer, Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Belgium

In 2002, Swedish researchers discovered that within certain foods, significant levels of acrylamide, a probable human carcinogen formed during heating processes such as frying, roasting or baking. Free asparagine and reducing sugars were indicated as the most important precursors. Over the past few years, significant progress has been made to reduce acrylamide formation.

Liquid Chromatography-Mass Spectrometry in food analysis

Issue 1 2008, Past issues  •  28 February 2008  •  Dr. Mark Buecking, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Germany

The determination of organic trace compounds in food analysis is of major importance for food quality and food safety aspects. Both the separation of the analyte from potential inferences in the food matrix, as well as the qualitative and quantitative determination of the target compound, are vital steps in analytical food chemistry.

Mycotoxin determination in foodstuffs

Issue 1 2007, Past issues  •  7 March 2007  •  S. Monbaliu, S. De Saeger and C. Van Peteghem, Ghent University, Laboratory of Food Analysis

This article focuses on the main principle of the liquid chromatographic tandem mass spectrometric (LC-MS/MS) determination of mycotoxins in foodstuffs. It also provides an overview of recent developments in mycotoxin analysis.

LC-MS/MS for safer seafood

Issue 2 2005, Past issues  •  3 May 2005  •  Kevin J. James, Mary Lehane, Brett Hamilton, Ambrose Furey, PROTEOBIO, Mass Spectrometry Centre for Proteomics and Biotoxin Research, Department of Chemistry, Cork Institute of Technology

Toxin contamination has forever been the curse on shellfish production worldwide. Dr Kevin J. James demonstrates how new technology can protect the health of shellfish lovers.

PROTEOBIO is at the forefront of food-borne biotoxin research in Europe and specialises in the development of novel methods to address the complex questions affecting food safety. The technologies used at PROTEOBIO to investigate biotoxins include liquid chromatography-mass spectrometry (LC-MS), incorporating nanotechnologies, to seek solutions to problems related to trace contamination of foods by potent bioactive compounds that can seriously impact on human health. Some of the key activities and interests pursued at PROTEOBIO are summarised in Figure 1. The theme of this article however, is the centre’s approach to the investigation of toxins in edible bivalve molluscs, including mussels, scallops and oysters.

 

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